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Understanding the intricate details of the interactions between membranes and membrane bound compartments is a challenging task. Electronmicroscopy using immunological methods together with genetic approaches and molecular studies of reconstituted systems are beginning to provide the needed framework of information. This chapter focuses on some of these processes
Since the invention of the light microscope and our first vistas of cells, technological and conceptual developments have gone hand in hand. Hence a review of some of the methodology is useful, although in the chapters that follow, results and techniques are frequently considered together. Our review first examines the techniques more traditionally associated with cell biology: microscopy an…