The basophil activation test (BAT) in bakers with obstructive allergic airway disease | Perpustakaan Fakultas Kedokteran dan Ilmu Kesehatan

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The basophil activation test (BAT) in bakers with obstructive allergic airway disease

SIMONIS, BETTINA - Nama Orang;

Abstract Background: e basophil activation test (BAT) represents a sensitive test method that is available for the detection of sensitization to rye and wheat our in bakers with obstructive allergic airway disease. Since ImmunoCAP® failes to detect specic immunoglobulin E (IgE) in a part of the investigat- ed bakers with airway disease, BAT should be used as an additional research method. Methods: A collective of 248 bakers taking part in the prevention program of the German Social Ac- cident Insurance Institution for the foodstus and catering industry (Berufsgenossenscha Nahrungsmittel und Gastgewerbe, BGN), as well as 31 non-allergic BGN employees as control subjects,
were investigated. BAT was performed according to the manufacturers’ instruction and reagents (Beckman Coulter, Krefeld, Germany). Activated baso- phils were stained with CD203c-PE and visualized using ow cytometry. e percentage of basophils that show signicantly higher CD203c expression following allergen-specic stimulation with rye and wheat our compared with unstimulated basophils yields the stimulation index (SI), which is considered as an positive allergic reaction from a value of
2.0. Total IgE and specic IgE to rye and wheat our were detected using the ImmunoCAP® FEIA
system (ermo Fisher Scientic). Results: In total, 7 % of aected bakers and 10 % of controls were non-responders. Using BAT, 83 % of „responder bakers“ were positive for either wheat or rye our. Control subjects were negative for both wheat and rye our. is yielded a sensitivity of 83 % and a specicity of 100 % for BAT stimulation related to the control group. Of the our-negative bak- ers in the CAP-FEIA, 38 % tested positive to our in the BAT. Conclusions: BAT is a method well suited as an adjunct to the serological diagnosis of allergic baker‘s asthma and rhinitis, particularly if the results of serological specic IgE detection are inconclusive in relation to the patient history.

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Judul Seri: -
No. Panggil: Artikel
Penerbit: German : Springer., 2016
Deskripsi Fisik: -
Bahasa: English
ISBN/ISSN: DOI: 10.1007/s40629-
Klasifikasi: NONE
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: Allergo J Int 2016; 25: 124–30
Subjek: Medicine
Allergic
airway disease
Info Detail Spesifik: -
Pernyataan Tanggungjawab: -

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The basophil activation test (BAT) in bakers with obstructive allergic airway disease

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