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Mutant prevention concentration of colistin alone and in combination with rifampicin for multidrug-resistant Acinetobacter baumannii

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Abstract Colistin-susceptible isolates of Acinetobacter baumannii often contain subpopulations that are resistant to colistin.Monotherapywithcolistincanleadtoselectivegrowth of these subpopulations and emergence of colistin-resistant strains.Ourobjectivesweretoexplorethesusceptibilitypattern of colistin-resistant subpopulations and investigate if combiningcolistinwithasecondantibioticcouldpreventtheirselective growth. Four colistin-susceptible clinical isolates of A. baumannii and one reference isolate were used. The mutant prevention concentration (MPC) of colistin, i.e. the concentration required to block growth of all single step-mutant subpopulations,wasdeterminedbyplatinganinoculumof109CFUon Mueller Hinton agar (MHA) plates containing 2-fold dilutions of colistin (0.125–128 mg/L). Susceptibility testing of colistinresistant subpopulations, obtained in the MPC assay, was performed with Etest. The MPC of colistin, in combination with rifampicin,wasdeterminedbyplatinganinoculumof109 CFU onMHA platescontainingcolistin(0.125–128mg/L)andfixed concentrations of rifampicin (1.1 mg/L or 4.4 mg/L). The colistin-resistant subpopulations demonstrated increased susceptibility to a number of agents compared to their main populations. These subpopulations were even susceptible to agents thatnormallyareinactiveagainstgram-negativebacteriaandall had rifampicin MICs of

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Informasi Detail

Judul Seri

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No. Panggil

artikel

Penerbit

USA : Springer., 2016

Deskripsi Fisik

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Bahasa

English

ISBN/ISSN

DOI 10.1007/s10096-0

Klasifikasi

NONE

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

Eur J Clin Microbiol Infect Dis (2016) 35:1845–185

Subjek

Medicine
prevention concentration

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Pernyataan Tanggungjawab

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